Di-tripeptides and oligopeptides are taken up via distinct transport mechanisms in Lactococcus lactis
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چکیده
منابع مشابه
Di-tripeptides and oligopeptides are taken up via distinct transport mechanisms in Lactococcus lactis.
Lactococcus lactis ML3 possesses two different peptide transport systems of which the substrate size restriction and specificity have been determined. The first system is the earlier-described proton motive force-dependent di-tripeptide carrier (E. J. Smid, A. J. M. Driessen, and W. N. Konings, J. Bacteriol. 171:292-298, 1989). The second system is a metabolic energy-dependent oligopeptide tran...
متن کاملSpecificity of peptide transport systems in Lactococcus lactis: evidence for a third system which transports hydrophobic di- and tripeptides.
A proton motive force-driven di-tripeptide carrier protein (DtpT) and an ATP-dependent oligopeptide transport system (Opp) have been described for Lactococcus lactis MG1363. Using genetically well-defined mutants in which dtpT and/or opp were inactivated, we have now established the presence of a third peptide transport system (DtpP) in L. lactis. The specificity of DtpP partially overlaps that...
متن کاملOligopeptides are the main source of nitrogen for Lactococcus lactis during growth in milk.
The consumption of amino acids and peptides was monitored during growth in milk of proteinase-positive (Prt+) and -negative (Prt-) strains of Lactococcus lactis. The Prt- strains showed monophasic exponential growth, while the Prt+ strains grew in two phases. The first growth phases of the Prt+ and Prt- strains were in same, and no hydrolysis of casein was observed. Also, the levels of consumpt...
متن کاملManipulation of activity and orientation of membrane-reconstituted di-tripeptide transport protein DtpT of Lactococcus lactis.
The di-tripeptide transport system (DtpT) of Lactococcus lactis was purified to apparent homogeneity by pre-extraction of crude membrane vesicles with octaethylene glycol monodecyl ether (C10E8), followed by solubilization with n-dodecyl-beta-D-maltoside (DDM) and chromatography on a Ni-NTA resin. The DtpT protein was reconstituted into detergent-destabilized preformed liposomes prepared from E...
متن کاملMolecular Mechanisms of Stress Resistance in Lactococcus lactis
Lactococcus is an economically important starter culture bacterium extensively used in the manufacture of the both soft and hard cheeses. During its growth and storage, and throughout cheese processing, it encounters a variety of stresses including osmotic, oxidative, temperature, acid and cell envelope stress. These stressors can cause damage to DNA, proteins, lipid membranes and peptidoglycan...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1993
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.175.7.2052-2059.1993